Table 2. Cysteine mutants in rP2X2 receptor.

Clone	I basal (pA/pF)	n	Clone	I basal (pA/pF)	n	Clone	I basal (pA/pF)	n
rP2X2R-T	72.2 ± 10.9	20	Q37C with			F44C with
G30C with			G342C	17.2 ± 2.5	19	I332C	69.9 ± 6.6	6
I328C*	N.T.		V343C	14.2 ± 1.4	5	L334C	40.6 ± 2.6	6
N333C*	N.T.		G344C	12.6 ± 1.8	8	A337C	28.8 ± 1.7	6
T336C*	N.T.		S345C	15.5 ± 3.5	8	I328C*	N.T.
S345C	76.4 ± 14.0	7	I328C*	N.T.		T336C*	N.T.
I351C*	N.T.		N333C*	N.T.		L338C*	N.T.
L352C*	N.T.		T336C*	N.T.		N333C*	N.T.
H33C with			L338C*	N.T.		Y47C with
I341C	70.2 ± 11.9	8	I40C with			P329C	34.5 ± 2.3	6
G342C	53.9 ± 12.9	7	L334C	119.9 ± 12.2	5	N333C	123.8 ± 10.3	8
S345C	95.9 ± 12.3	30	L338C	135.4 ± 13.1	9	V48C with
L347C	157.6 ± 21.2	6	S345C	130.3 ± 18.5	8	I328C	12.8 ± 1.8	40
C348	65.1 ±17.5	12	L41C with			P329C	22.8 ± 4.9	6
R34C with			L334C	50.3 ± 11.4	10	I332C	72.2 ± 10.2	6
G344C	5.8 ± 0.6	27	L338C	44.4 ± 9.5	6	N333C*	N.T.
S345C	11.6 ± 2.4	8	Y43C with			T336C*	N.T.
M35C with			I328C	4.3 ± 0.5	12	F49C with
S345C	76.3 ± 11.2	10	I332C	13.1 ± 1.2	6	I332C	71.8 ± 8.9	5
V36C with			N333C	85.9 ± 7.4	13	V51C with
S345C	81.7 ± 4.9	6	T336C	2.7 ± 0.7	5	I328C	57.6 ± 5.8	6
Q37C with			S340C	34.9 ± 8.8	45	S54C with
S340C	107.6 ± 14. 8	6	G344C	5.6 ± 0.2	6	I328C	22.5 ± 4.0	5

The double mutations with asterisks are from previous studies [20], [21], which demonstrated that none of the double mutations formed disulfide bonds. N.T. means this double mutation was not tested. Data shown in the table are the mean ± S.E.M. from the cells studied, and the number of cells studied is given by n.