Table 2. Cysteine mutants in rP2X2 receptor.
Clone | I basal (pA/pF) | n | Clone | I basal (pA/pF) | n | Clone | I basal (pA/pF) | n |
rP2X2R-T | 72.2 ± 10.9 | 20 | Q37C with | F44C with | ||||
G30C with | G342C | 17.2 ± 2.5 | 19 | I332C | 69.9 ± 6.6 | 6 | ||
I328C* | N.T. | V343C | 14.2 ± 1.4 | 5 | L334C | 40.6 ± 2.6 | 6 | |
N333C* | N.T. | G344C | 12.6 ± 1.8 | 8 | A337C | 28.8 ± 1.7 | 6 | |
T336C* | N.T. | S345C | 15.5 ± 3.5 | 8 | I328C* | N.T. | ||
S345C | 76.4 ± 14.0 | 7 | I328C* | N.T. | T336C* | N.T. | ||
I351C* | N.T. | N333C* | N.T. | L338C* | N.T. | |||
L352C* | N.T. | T336C* | N.T. | N333C* | N.T. | |||
H33C with | L338C* | N.T. | Y47C with | |||||
I341C | 70.2 ± 11.9 | 8 | I40C with | P329C | 34.5 ± 2.3 | 6 | ||
G342C | 53.9 ± 12.9 | 7 | L334C | 119.9 ± 12.2 | 5 | N333C | 123.8 ± 10.3 | 8 |
S345C | 95.9 ± 12.3 | 30 | L338C | 135.4 ± 13.1 | 9 | V48C with | ||
L347C | 157.6 ± 21.2 | 6 | S345C | 130.3 ± 18.5 | 8 | I328C | 12.8 ± 1.8 | 40 |
C348 | 65.1 ±17.5 | 12 | L41C with | P329C | 22.8 ± 4.9 | 6 | ||
R34C with | L334C | 50.3 ± 11.4 | 10 | I332C | 72.2 ± 10.2 | 6 | ||
G344C | 5.8 ± 0.6 | 27 | L338C | 44.4 ± 9.5 | 6 | N333C* | N.T. | |
S345C | 11.6 ± 2.4 | 8 | Y43C with | T336C* | N.T. | |||
M35C with | I328C | 4.3 ± 0.5 | 12 | F49C with | ||||
S345C | 76.3 ± 11.2 | 10 | I332C | 13.1 ± 1.2 | 6 | I332C | 71.8 ± 8.9 | 5 |
V36C with | N333C | 85.9 ± 7.4 | 13 | V51C with | ||||
S345C | 81.7 ± 4.9 | 6 | T336C | 2.7 ± 0.7 | 5 | I328C | 57.6 ± 5.8 | 6 |
Q37C with | S340C | 34.9 ± 8.8 | 45 | S54C with | ||||
S340C | 107.6 ± 14. 8 | 6 | G344C | 5.6 ± 0.2 | 6 | I328C | 22.5 ± 4.0 | 5 |
The double mutations with asterisks are from previous studies [20], [21], which demonstrated that none of the double mutations formed disulfide bonds. N.T. means this double mutation was not tested. Data shown in the table are the mean ± S.E.M. from the cells studied, and the number of cells studied is given by n.