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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: J Immunol. 2013 Jul 3;191(4):1606–1613. doi: 10.4049/jimmunol.1301116

Figure 6. Notch-deprived CD4+ T cells have markedly reduced IL-17A and IFNγ production in the CNS despite preserved T-bet expression.

Figure 6

Spinal cord infiltrates from immunized BM chimeras restimulated for 6 hours with anti-CD3/CD28. (A) Frequency and number of IFNγ and IL-17A-producing DN CD4+ T cells in the DLN or CNS by intracellular flow cytometry (n=4–5 mice/group; 2 experiments). Markedly decreased production of IFNγ and reduced production of IL-17 was observed in CD45.2+ DNMAML T cells in the CNS; (B) Concomitant expression of T-bet and IFNγ by DN CD4+ T cells in the DLN or CNS as assessed by intracellular flow cytometry (n=4–5 mice/group; 2 experiments). T-bet induction was preserved in all compartments. IFNγ production by Notch-deprived DN T cells was reduced in the CNS but not in the DLN (as revealed by the %T-bet+ cells expressing IFNγ). p<0.05*; p<0.01**; p<0.001***.