Fig. 4.

Effect of recombinant Mst on the adipogenic differentiation in primary cultures of WT and Mst KO MEFs. Cells were plated on a six-well plate and allowed to differentiate under adipogenic conditions either in absence or presence of recombinant Mst (2μg/ml) as described in materials and methods. Cells were fixed and stained with Oil-Red O (A) and quantitative analysis of the staining (B) is presented as IOD (area x intensity of staining). (C) Real-time quantitative PCR analysis of Cidea, FGF21 and Elov2 in WT and Mst KO MEFs undergoing BAT-specific differentiation. Experiment was performed three times and representative data is shown (**, denotes p≤0.005).