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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Gene Ther. 2013 Jan 31;20(8):807–815. doi: 10.1038/gt.2013.1

Figure 1. Common mutations following directed evolution of VSV-G.

Figure 1

(a) The frequency of mutation at each amino acid residue of VSV-G among 36 randomly chosen VSV-G clones after 5 or 6 selection steps. (b) The location of each apparent ‘hot spot mutation’ in the crystal structure of the prefusion form of VSV-G (PDB ID: 2J6J). Figure was made using PyMol (http://www.pymol.org). Each monomer of VSV-G was colored in green, purple, and sky-blue, respectively. Green, blue, and red balls represent carbon, nitrogen, and oxygen atoms, respectively.