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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: J Immunol. 2013 Jul 5;191(4):1948–1956. doi: 10.4049/jimmunol.1202092

Figure 2. Primary vascularization of allografts is associated with lack of inflammatory T cell indirect alloreactivity.

Figure 2

The frequencies of T cells activated either via direct (panel A and C) or indirect allorecognition (panel B and D) and secreting pro-inflammatory cytokines type 1 cytokines were measured by ELISPOT. Alloresponses were measured using spleen T cells collected from naïve C3H mice (no Tx) and from C3H mice transplanted (d10 POD) with either conventional (non-vascularized, NV-skin) or primarily vascularized (V-skin) fully allogeneic B6 skin allografts (panel A and B). Panel C and D show direct and indirect alloresponses recorded in C3H mice transplanted with a typical vascularized B6 heart (V-heart) or a non-vascularized B6 heart (NV-heart). The results are expressed as spots per million T cells for each cytokine ± SD. 5-8 mice were tested in each group.