Table 3.
Effects of chronic (10-day) intermittent escalating-dose heroin administration, 12-hour withdrawal, and 10-day withdrawal with heroin challenge on mRNA levels (attomole/μg total RNA) of arginine vasopressin (AVP) and corticotropin-releasing factor (CRF) in the medial hypothalamus (A), and AVP V1b type receptor (V1b) and CRF type I receptor (CRF-R1) in the anterior pituitary (B).
| A. | |||
|---|---|---|---|
| Experiment | Treatment | AVP | CRF |
| I. 12-hour withdrawal with heroin challenge | Chronic saline + Sal | 103 ± 11 | 1.13 ± 0.09 |
| Chronic saline + HC | 104 ± 9 | 1.10 ± 0.15 | |
| Chronic heroin + Sal | 112 ± 9 | 1.30 ± 0.10 | |
| Chronic heroin + HC | 77 ± 8 | 0.94 ± 0.11 | |
| II. 10-day withdrawal with heroin challenge | Saline withdrawal + Sal | 93 ± 9 | 1.06 ± 0.18 |
| Saline withdrawal + HC | 90 ± 7 | 1.10 ± 0.09 | |
| Heroin withdrawal + Sal | 94 ± 15 | 1.13 ± 0.19 | |
| Heroin withdrawal + HC | 99 ± 9 | 1.11 ± 0.12 | |
| B. | |||
|---|---|---|---|
| Experiment | Treatment | V1b | CRF-R1 |
| I. 12-hour withdrawal with heroin challenge | Chronic saline + Sal | 1.7 ± 0.19 | 0.92 ± 0.10 |
| Chronic saline + HC | 1.8 ± 0.11 | 0.95 ± 0.18 | |
| Chronic heroin + Sal | 1.9 ± 0.34 | 1.20 ± 0.25 | |
| Chronic heroin + HC | 2.2 ± 0.41 | 1.11 ± 0.30 | |
| II. 10-day withdrawal with heroin challenge | Saline withdrawal + Sal | 1.9 ± 0.19 | 0.89 ± 0.16 |
| Saline withdrawal + HC | 2.0 ± 0.29 | 0.88 ± 0.27 | |
| Heroin withdrawal + Sal | 1.9 ± 0.10 | 0.98 ± 0.33 | |
| Heroin withdrawal + HC | 1.9 ± 0.26 | 0.92 ± 0.31 | |
See details in Table 1. Data shown in table are treatment group mean ± SEM (n=6-9).