Figure 4. LLOMe-mediated caspase-1 depletion is independent of Nlrp3 signaling. Wild-type and Nalp3/Asc/caspase-1-deficient C57BL/6 macrophages were primed with 250 ng/ml LPS in the presence or absence of 100 μM CA-074-Me (CAMe) for 2 h. Cells were then challenged with 2.5 mM LLOMe or 10 mM nigericin for 2 h, and cell death was measured using PI exchange assays (top row). Cellular lysates were examined for levels of pro-caspase-1 and actin by immunoblotting (bottom row).