Figure 5. Cathepsin C controls LLOMe-mediated degradation of IL-1β. Wild type, cathepsin B-, C-, L- and S-deficient macrophages were primed with 250 ng/ml LPS for 2 h and then treated with increasing concentrations of LLOMe for 2 h. LLOMe-treated wild type macrophages were also tested in the presence 100 μM CA-074-Me. Cell death was measured by propidium iodide exclusion assay, and IL-1β levels were determined by immunoblotting from lysates. Representative experiment is shown, and PI measurements were performed in triplicates.