Figure 6. LLOMe triggers broad degradation of cellular proteins in macrophages. (A) 2D electrophoresis gel of proteins isolated from untreated, LLOMe- and nigericin-treated macrophages. C57BL/6-derived macrophages were primed with 250 ng/ml for 2 h, and then challenged with 2.5 mM LLOMe or 10 mM nigericin. Protein lysates were isolated from untreated, nigericin and LLOMe-treated macrophages 90 min post nigericin/LLOMe exposure. Protein samples were labeled with Cy2, Cy3 and Cy5, respectively, and separated on a 2D-DIGE gel. The red box on each gel highlights the low PI and low molecular weight region where the greatest variability is observed. (B) Bioinformatic prediction of the location of murine actin, pro-caspase-1, pro-IL-18 and pro-IL-1β on a 2D PAGE gel.