Figure 2. Tumor re-growth-delay curves for tumors created from two TNBC cell lines: 4T1 and 67NR cells using two different methods of caloric restriction. Mice were treated with each of the following conditions for 67NR (A) cell lines: ad libitum (AL) diet, radiation (RT), alternate day feeding (ADF) or ADF + RT, and their weights were recorded (B). From the reference of the AL cohort for 67NR tumors at 1,000 mm³, RT gave a 16% growth delay, ADF 14%, ADF + RT 25%. From the reference of the AL cohort for 4T1 and weights were tumors at 1,000 mm³ (C), RT gave a 23% growth delay, ADF 30%, ADF + RT 45% also recorded for this cohort (D). Using the 4T1 tumors, the ADF method was then compared with a 30% reduction in calories (CR) (E) using: AL, RT, CR and CR + RT. From the reference of the AL cohort for 4T1 tumors at 1,000 mm³, RT gave a 23% growth delay, CR 56%, CR+RT 82% with an average weight reduction (F) of 12%. Based on this, the optimal method for performing future studies would be a constant reduction of 30% of the caloric intake. *Denotes p < 0.05 by Student’s t-test at 1,000 mm³.