Figure 3. Molecular effects of treatment. Proliferation was evaluated by Ki-67 (A), and bcl-2 was used to evaluate apoptosis (B) for all conditions: Ad lib, CR, IR and CR + IR. Combination therapy was noted to decrease proliferation and increase apoptosis in tumors. qRT-PCR of IGF-1R pathway molecules shows downregulation (C) with either CR, IR or both. *Notes statistical significance with a p < 0.05.