Figure 4. Immunohistochemical investigation of proliferation and IGF-1R pathway. Proliferation was also evaluated by immunohistochemistry (IHC) with Ki-67 staining, which was noted to have higher proliferation in the ad libitum tumors (A) compared with the tumors treated with CR and IR. IGF-1R was noted to have positive staining (3+) in AL tumors and negative (1+) in CR + IR samples while the downstream target of GSK-3B revealed positive staining (3+) in AL tumors and 2+ staining in CR + IR tumors.