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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Biomol Screen. 2013 May 2;18(8):930–937. doi: 10.1177/1087057113483559

Fig. 3. Scatter plot analysis of the high-throughput LOPAC pilot screen.

Fig. 3

(a) All data from all assay plates (n=17, triplicate results), including controls, are displayed. Large separation in activity (Z’ =0.85 ± 0.05, S/B=7.3 ± 0.6) between wells dosed with SR121463 (“high control”) and DMSO (“low control”), as well as a broad distribution of hits indicate an excellent HTS assay. (b) Representative graph of compound activity correlating plate replicas. The best fit line has an r2=0.86, indicative of the high fidelity of the no-wash assay hit identification.