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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Eur J Neurosci. 2013 Apr 16;38(3):2477–2490. doi: 10.1111/ejn.12202

Figure 4. Immunoblot analysis of protein expression of glutamate transporters reveals that GLT-1 and EAAC1 was significantly decreased in the striatum of 11 week-old R6/2; GLT-1+/− mice compared to age-matched R6/2; GLT-1+/+ mice.

Figure 4

A - Immunoblot analysis using nGLT-1, GLT-1a, GLT-1b, EAAC1, GLAST and β-tubulin antibodies on lysates from the striatum of 11 week old GLT-1+/+, GLT-1+/−, R6/2; GLT-1+/+ and R6/2; GLT-1+/− mice. B Quantification of the protein expression of total GLT-1, GLT-1a, GLT-1b, EAAC1 and GLAST expression in the striatum of GLT-1+/+, GLT-1+/−, R6/2; GLT-1+/+ and R6/2; GLT-1+/− mice. Histograms showing the average absolute optical density, indicative of protein expression of total GLT-1, GLT-1a, GLT-1b, EAAC1 and GLAST in the striatum of three 11 week old GLT-1+/+ (solid black), GLT-1+/− (spotted), R6/2; GLT-1+/+(striped) and R6/2; GLT-1+/− mice (solid white). *P< 0.05, **P <0.01, ***P < 0.001 (One-way ANOVA followed by Bonferroni’s post hoc test); ±SD; n=3.