A - Immunoblot analysis using nGLT-1, GLT-1a, GLT-1b, EAAC1, GLAST and β-tubulin antibodies on lysates from the striatum of 11 week old GLT-1+/+, GLT-1+/−, R6/2; GLT-1+/+ and R6/2; GLT-1+/− mice. B Quantification of the protein expression of total GLT-1, GLT-1a, GLT-1b, EAAC1 and GLAST expression in the striatum of GLT-1+/+, GLT-1+/−, R6/2; GLT-1+/+ and R6/2; GLT-1+/− mice. Histograms showing the average absolute optical density, indicative of protein expression of total GLT-1, GLT-1a, GLT-1b, EAAC1 and GLAST in the striatum of three 11 week old GLT-1+/+ (solid black), GLT-1+/− (spotted), R6/2; GLT-1+/+(striped) and R6/2; GLT-1+/− mice (solid white). *P< 0.05, **P <0.01, ***P < 0.001 (One-way ANOVA followed by Bonferroni’s post hoc test); ±SD; n=3.