Figure 4.
Comparison of mRNP movement on single actin filaments versus bundles. Representative run length histograms of Myo4p mRNPs containing 1 zipcode (E1) or 4 zipcodes on (a) single actin filaments or (b) actin bundles. Both tracks have Tpm1p bound. Asterisk (*) indicates run lengths that are significantly different between filaments and bundles with P < 0.001 (Kolmogorov-Smirnov Test; n ≥ 103). (c) Run frequencies of Myo4p mRNP motility containing 1 (E1) or 4 zipcodes on single actin filaments or bundles. The run frequency (number of runs per μM Myo4p per μm actin per sec) with single actin filaments is normalized to one. (d) Speed distributions of Myo4p mRNPs on single actin filaments and bundles. The mean speed is indicated with a red line. Error is in s.e.m; n ≥ 103. Conditions: 140 mM KCl, pH 7.4, 1 mM MgATP