Fig. 4.
Interaction of naturally occurring fatty acids and fatty acyl-CoA with mPPARα. Direct binding assay based on quenching of mPPARα aromatic amino acid fluorescence emission when titrated with the following ligands: (A) palmitic acid, (B) stearic acid, (C) oleic acid, (D) EPA, (E) palmitoyl-CoA, (F) stearoyl-CoA, (G) oleoyl-CoA, (H) EPA-CoA, (I) lauric acid, (J) lauryl-CoA, (K) clofibrate, and (L) rosiglitazone. Data are presented as the change in fluorescence intensity (F0−Fi) plotted as a function of ligand concentration. Insets represent linear plots of the binding curve from each panel. All values represent mean ± SE, n ≥ 3.