TABLE 3.
Effect of ligands on the relative proportion of hPPARα secondary structure determined by CD
| Average | Total H ± SE | Total S ± SE | Trn ± SE | Unrd ± SE |
| hPPARα | 32 ± 1 | 19 ± 1 | 21.3 ± 0.3 | 29.3 ± 0.5 |
| hPPARα + lauric acid | 30 ± 1 | 20 ± 2 | 21.8 ± 0.4 | 28.7 ± 0.3 |
| hPPARα + lauryl-CoA | 31 ± 3 | 18.2 ± 0.2 | 20 ± 1 | 29 ± 1 |
| hPPARα + palmitic acid | 16 ± 3** | 32 ± 2** | 21.7 ± 0.4 | 30 ± 1 |
| hPPARα + palmitoyl-CoA | 13 ± 3** | 34 ± 2** | 22.5 ± 0.2 | 30 ± 1 |
| hPPARα + palmitoleic acid | 22 ± 4* | 28 ± 3* | 21 ± 1 | 28 ± 1 |
| hPPARα + palmitoleoyl-CoA | 24 ± 5# | 27 ± 3* | 21 ± 1 | 29 ± 1 |
| hPPARα + stearic acid | 14 ± 3** | 33 ± 2** | 22.0 ± 0.2 | 31 ± 2 |
| hPPARα + stearyl-CoA | 24 ± 4# | 27 ± 2* | 21 ± 1 | 29 ± 1 |
| hPPARα + oleic acid | 18 ± 2** | 31 ± 2** | 22 ± 1 | 29 ± 1 |
| hPPARα + oleoyl-CoA | 26 ± 3 | 25 ± 2# | 21 ± 1 | 28.3 ± 0.3 |
| hPPARα + linoleic acid | 27 ± 6 | 28 ± 2* | 19 ± 2* | 26 ± 3 |
| hPPARα + linoleoyl-CoA | 24 ± 3# | 26 ± 2* | 21 ± 1 | 28.8 ± 0.1 |
| hPPARα + arachidonic acid | 19 ± 1* | 30 ± 1** | 21.8 ± 0.3 | 28.9 ± 0.1 |
| hPPARα + arachidonoyl-CoA | 30 ± 1 | 23.4 ± 0.4 | 19.4 ± 0.5# | 26.9 ± 0.4 |
| hPPARα + EPA | 14 ± 7** | 24 ± 6 | 23 ± 2 | 33 ± 5 |
| hPPARα + EPA-CoA | 21 ± 1* | 29 ± 1* | 21.6 ± 0.3 | 29 ± 1 |
| hPPARα + DPA | 17 ± 4** | 32 ± 3** | 21.9 ± 0.1 | 30 ± 1 |
| hPPARα + DPA-CoA | 20 ± 1* | 30 ± 1** | 21 ± 1 | 29.6 ± 0.2 |
| hPPARα + DHA | 12 ± 3** | 38 ± 4** | 21 ± 1 | 30 ± 1 |
| hPPARα + DHA-CoA | 20 ± 2* | 29 ± 2* | 22 ± 1 | 28.9 ± 0.2 |
| hPPARα + clofibrate | 33 ± 1 | 15 ± 1* | 22 ± 1 | 30 ± 1 |
| hPPARα + rosiglitazone | 29 ± 1 | 22 ± 2 | 20 ± 1 | 28 ± 1 |
Structure abbreviations: H, total helices (sum of regular α-helices and distorted α-helices); S, total sheets (sum of regular β-sheets and distorted β-sheets); Trn, β-turns; Unrd, unordered. Asterisks represent significant differences between hPPARα only and hPPARα in the presence of added ligand. *P < 0.05, **P < 0.001, #P = 0.07.