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SDS-PAGE and Western immunoblot of recombinant DesC. A: SDS-PAGE. B: Western blot. M, protein marker; 1, crude extract of E. coli BL21(DE3)RIL after expression of recombinant DesC-ST; 2, purified recombinant DesC-ST after Strep-Tactin affinity chromatography.
