Fig. 1.

Bile acids and synthetic FXR ligand GW4064 increase human ADH1 mRNA and protein levels. Human primary hepatocytes (A) and HepG2 cells (B) were incubated with vehicle (Control), 50 μM CDCA or 5 μM GW4064 for 24 h. Total RNA was extracted and reverse transcribed for analysis by real-time quantitative PCR. Specific mRNA levels normalized to 18S content are expressed relative to controls, which were set as 1 (mean ± SEM). C: Western blot analysis of total protein lysates from Huh7 cells treated as in (A). Protein signals were quantified and pan ADH1 levels were normalized to actin content. *P < 0.05; **P < 0.01; ***P < 0.001 versus untreated controls. The results are representative of three independent experiments from triplicate dishes.