Fig. 5.

Cholesterol absorption into C57 and FVB mouse intestinal cells following olive oil gavage. Nonfasted female C57 and FVB mice were injected retro-orbitally with 100 μl tyloxapol (10% v/v) followed by gavage with 2 μCi [³H]cholesterol in 200 μl olive oil. After 7 h, the small intestine was harvested and divided into four equal-length segments. Each segment was washed with 5 ml PBS to remove luminal contents, dissolved in 1 ml NaOH, and tissue [³H]cholesterol levels measured by liquid scintillation counting. Segment I1 is closest to the stomach. Significant differences between strains are represented by * P < 0.01.