Fig. 6.

Cholesterol absorption and secretion from C57 and FVB mouse primary enterocytes. A: Enterocytes from intestinal segments I1 and I2 harvested from nonfasted female C57 and FVB mice were incubated with bile salt micelles containing [³H]cholesterol for various times, and the cell-associated [³H]cholesterol levels were measured at several time points, as described in Materials and Methods. B: Nonfasted C57 and FVB mice were gavaged with 2 μCi [³H]cholesterol in 200 μl olive oil. After 3 h, enterocytes from intestinal segments I1 and I2 were washed, harvested, and plated in DMEM, and secretion of the cell-associated [³H]cholesterol levels were measured at several time points, as described in Materials and Methods. Data represent mean ^+/− standard deviation of enterocytes from triplicate mice. P values compare C57 I2 to FVB I2.