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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: J Immunol. 2013 Apr 15;190(10):5030–5036. doi: 10.4049/jimmunol.1203325

Figure 4. Btn2a2 binding interferes with activation of the PI3K/Akt pathway in anti-CD3 and anti-CD28-activated T cells.

Figure 4

CD3+ T cells stimulated with 1 μg/ml anti-CD3, 1 μg/ml anti-CD28 and 10 μg/ml fusion protein as indicated. (A) Samples were immunoprecipitated with anti-p85 antibody, treated as in 3, and phosphorylation analysed using a p-p85 antibody. p85 antibody (total-p85) controlled loading. (B) Blotted proteins were analysed for phosphorylation of Akt using antibodies (p-Akt (Ser473) or p-Akt (Tyr308)). Antibody to total Akt (Akt) - sample loading. Data representative of 3 experiments.