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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: J Immunol. 2013 Mar 18;190(8):4349–4359. doi: 10.4049/jimmunol.1203266

Figure 4.

Figure 4

PhagoSight analysis confirms that CXCL8s morphants display a reduced zebrafish neutrophil recruitment in acute inflammation. Tailfins of Tg(mpx:gfp)i114 previously microinjected with MO StdC, MO cxcl8-l1 and MO cxcl8-l2 were transected at 3 dpf and time-lapse movies were performed under a wide field fluorescence microscope (MO StdC=270 tracks, MO cxcl8-l1=147 tracks and MO cxcl8-l2=128 tracks from n=10 larvae as 3 independent experiments) and further analyzed by PhagoSight. Distinct parameters were determined to study neutrophil migratory behavior namely: (A) Total Number of Tracks; (B) Number of Tracks Entering the Wound; (C) Ratio of Number of Tracks in Wound/Total Number of Tracks; (D) Number of Tracks Cross the Wound and Back; (E) Tracks Enter the Wound time; (F) Number of Hops in Wound. Longer horizontal bars represent the means and shorter horizontal bars represent SEM. P values were calculated using one-way ANOVA and Bonferroni multiple comparison test, *P<0.05, **P<0.01, and ***P<0.001.