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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2013 Feb 25;190(7):3216–3224. doi: 10.4049/jimmunol.1202926

Figure 3. B27 dimers and free heavy chains stimulate KIR3DL2CD3ε reporter T cells more strongly than control HLA class I.

Figure 3

A. IL-2 production by KIR3DL2CD3ε–transduced T cells stimulated with recombinant HLA-B27 dimer and -B27 or HLA-A3 heterotrimers. Results, presented as mean values from triplicate samples (pg/ml)±1SD. B IL-2 secretion by KIR3DL2CD3ε–transduced Jurkat T cells, stimulated with parental LBL.721.221 (221) cells and cells transfected with HLA-B27, HLA-B27C67S, HLA-B7, HLA-B35, HLA-A2 and HLA-A3. Results presented as mean values from 5 independent experiments (pg/ml) ± 1SEM. C. IL-2 production by KIR3DL2CD3ε–transduced T cells stimulated with 221B27 cells with HC10, W632, ME1 and IgG2a and IgG1 isotype control MAbs or the anti-KIR3DL2 MAb (DX31). Results presented as mean values from triplicate samples (pg/ml) ± 1SD are representative of 4 independent experiments. D. IL-2 production by KIR3DL2CD3ε transduced T cells stimulated with parental LBL.221.220 cells, 220B8, 220B27 cells with HC10, W632, ME1 and IgG2a and IgG1 isotype control MAbs and 220B27 cells transfected with human tapasin (HuTPN). Results presented as mean values from triplicate samples (pg/ml) ± 1SD are representative of 4 independent experiments.