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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2013 Feb 25;190(7):3216–3224. doi: 10.4049/jimmunol.1202926

Figure 4. B cell lines and monocytes from B27+SpA patients and B27+controls express HLA-class I ligands for KIR3DL2.

Figure 4

A Representative FACS stain with W632, HC10 and ME1 antibodies of an EBV transformed B cell line from a B27+SpA patient. Representative stain from one of four different B cell lines. Geometric MFIs for staining with HC10, ME1 and W632 were 406, 1443 and 3464 respectively. B Representative FACS stain with HC10 and ME1 antibodies of CD14+ monocytes from a B27+SpA patient. Representative stain from one of four monocyte samples. Geometric MFIs for staining with HC10, ME1 and W632 were 82, 1315 and 2724 respectively. C IL-2 production by KIR3DL2CD3ε reporter cells stimulated with primary B cell lines and D primary monocyte lines from a B27+ healthy control and a B27+ AS patient. IL-2 production by reporter cells is inhibited by anti-heavy chain (HC10), -KIR3DL2 and W632 monoclonals. Representative data from experiments with 4 different B cell lines and 3 monocyte lines. Results with B cell lines were repeated on 3 independent occasions. Results are expressed as mean values of IL-2 pg/ml +/− 1SD. * values P<0.05 by ANOVA.