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. Author manuscript; available in PMC: 2013 Aug 7.

Published in final edited form as: Dev Biol. 2011 Sep 24;360(1):195–207. doi: 10.1016/j.ydbio.2011.09.021

Fig. 3 — PUF-8 regulates transgene expression mediated by 3′ UTRs of several germline mRNAs. Fluorescence photomicrographs of distal part of C. elegans adult hermaphrodite gonad. In (A) and (B), expression patterns of GFP::H2B under the control of the indicated 3′ UTRs in the wild-type and puf-8(RNAi) gonads are shown. (A) Negative regulation by PUF-8; (B) positive regulation by PUF-8. (C) Expression pattern of the GFP::PAL-1-pal-1 3′ UTR transgene in the indicated RNAi or genetic background are shown: puf-8(−/+) – heterozygous for the null allele, ok302; puf-8(−/−) – homozygous for ok302. (D) Expression pattern of ubc-6 3′ UTR transgene in the indicated RNAi background, which reveals the redundant control by FBF and PUF-8. (E) Expression pattern of GFP::PAL-pal-1 3′ UTR in mex-3(RNAi) gonad.

Fig. 3 — PUF-8 regulates transgene expression mediated by 3′ UTRs of several germline mRNAs. Fluorescence photomicrographs of distal part of C. elegans adult hermaphrodite gonad. In (A) and (B), expression patterns of GFP::H2B under the control of the indicated 3′ UTRs in the wild-type and puf-8(RNAi) gonads are shown. (A) Negative regulation by PUF-8; (B) positive regulation by PUF-8. (C) Expression pattern of the GFP::PAL-1-pal-1 3′ UTR transgene in the indicated RNAi or genetic background are shown: puf-8(−/+) – heterozygous for the null allele, ok302; puf-8(−/−) – homozygous for ok302. (D) Expression pattern of ubc-6 3′ UTR transgene in the indicated RNAi background, which reveals the redundant control by FBF and PUF-8. (E) Expression pattern of GFP::PAL-pal-1 3′ UTR in mex-3(RNAi) gonad.