Figure 1.

Endogenous A₃ARs aggregate into plaque-like microdomains that regulate processes involving cytoskeletal remodelling. (A) Representative plots showing directional migration of neutrophils within fMLP gradients is inhibited by A₃AR-selective antagonist MRS1334. (B) Quantification showing 51% reduction (***P<0.0001; Student’s t-test) of migration speed of MRS1334-treated cells (n=97–127 cells, three separate experiments). (C,D) Flow cytometry-based displacement experiments using CA200645 and unlabelled MRS1334 showed that the fluorescent ligand predominantly detected A₃ARs. Data shown are means±s.e.m. of three separate experiments. A₃AR, A₃-adenosine receptor; fMLP, fMet-Leu-Phe.