Figure 2.
A3AR activation promotes the extension of membrane projections from human neutrophils. (A) Confocal imaging of Vybrant DiO/CA200645-labelled human neutrophils revealed that A3AR plaques were frequently associated with membrane projections. Quantitative analysis of neutrophils isolated from the blood of three different healthy volunteers revealed that 69.3±6.7% of these projections were associated with A3AR plaques. (B) A Riveal Contrast Microscope (Quorum, Ontario, Canada) was used to show that these projections are also found on unstained, phorbol 12-myristate 13-acetate-treated human neutrophils. A3AR, A3-adenosine receptor.