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. 2013 Aug 7;3:2375. doi: 10.1038/srep02375

Figure 5. Expression profile of interferon sensitive genes (ISGs) and proteins in cancer cells and tumors.

Figure 5

(a) Semi-quantitate RT-PCR was used to determine the presence or absence of various ISG genes in LM-1 and EMT-6 cells, with or without exposure to conditioned media from RAW264.7 (RCM) or J774A.1 (JCM) or tumors grown in the respective syngeneic mice. Dosimetric measurements of the respective ISG bands in (b) LM-1 and (c) EMT-6 cells and tumors. Data represent fold increase from the respective control cells and was analyzed using Image J software. (d) Western blot analysis showing the presence or absence of two anti-viral proteins, OAS2 and Mx2, in the cells and tumors. Equivalent amount of protein (20 μg) was loaded for each lane. Blots shown in here were cropped using Photoshop software and the full-length blots are presented in Supplementary Fig. S1.