Figure 4.

IFN-β regulates TNF-α expression in peritoneal macrophages isolated from FVB mice. Peritoneal macrophages were untreated or stimulated with 2 nℳ of recombinant mouse IFN-β. At the indicated time, expression of IFN pathway response genes; STAT1 (a), NOS2 (b), cytokines TNF-α (c) or IL-12p40 (d) production were evaluated by quantitative reverse transcriptase-PCR. Peritoneal macrophages pretreated with 6.5 nℳ of a neutralizing antibody against mouse IFN-β were analyzed (1, 8 or 20 h) following the LPS exposure and analyzed for TNF-α (e) or IL-12p40 (f) production. Values represent the average±s.d. samples analyzed as duplicates. Representative data shown is from one of two independent experiments.