Figure 2.

Parenchymal descendants of Fgf10⁺ tanycytes express mature neural cell type markers and respond to acute fasting. A–D″, Expression of NeuN (A–B”), DARPP32 (D–D''), NPY (E, E'), and more rarely, GFAP (C, C') by parenchymal βgal⁺ cells, but not βgal⁺ tanycytes, in the hypothalamus of Fgf10^nlacZ mice. C, Side view of a three-dimensionally reconstructed image. E–E″, A subset of parenchymal βgal⁺ cells express the orexigenic neurotransmitter, NPY. E', E”, Cut views of a βgal⁺/NPY⁺ cell. F–G”, Upregulation of c-Fos expression and phosphorylated STAT3 in a subset of parenchymal βgal⁺ cells, in response to acute fasting alone (F–F”), or after administration of leptin to fasted mice (G–G”). Arc, Arcuate nucleus. Scale bars: A, C, 50 μm; C', E–G, 20 μm; B–B”, D-D”, F', F”, G', G”, 5 μm.