Figure 3.

Abundance and distribution of proliferating cells in the postnatal and adult hypothalamus. A, B, Cumulative exposure of P60 Fgf10^nlacZ mice to BrdU or EdU results in labeling of numerous cells in the hypothalamus with more cells being labeled after BrdU than EdU application. Most BrdU- or EdU-incorporating cells are found in the parenchymal compartment. C, A higher level of cell proliferation is observed in animals pulsed at P28–P32 (early post-weaning stage) compared with adult (P60–P70), with the difference relating mostly to parenchymally located cells than ependymal ME cells (where tanycytes reside). D, Comparable numbers of BrdU⁺/βgal⁺ tanycytes are found in P28–P32 and P60-pulsed animals (similar data were obtained with EdU; data not shown). Number of animals analyzed per condition is shown inside bars. *p < 0.05; **p < 0.01.