Figure 5.

Abundance and contribution of Fgf10⁺ tanycytes to hypothalamic parenchymal cells. A–I, Apparent descendants of Fgf10⁺ tanycytes, revealed by βgal immunolabeling, occupy the arcuate (Arc), ventromedial (VM), and to a lesser degree the dorsomedial (DM) and lateral (LH) hypothalamic nuclei at P60. J–N, Cross age comparison shows a conserved presence of such descendants in the arcuate nucleus. Note the reduction in the intensity of βgal label in parenchymal, but not ependymal (tanycytic) cells at P150 and P400. O–Q, Quantification of βgal⁺ cells in parenchymal and ependymal/ME compartments. Data point on each graph represent P4, P30, P60, P150, and P400, respectively. n = 3 for each age group. *p < 0.05; **p < 0.01. Scale bars: A–I, 75 μm; J–N, 50 μm.