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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Am J Trop Med Hyg. 2009 Dec;81(6):1030–1034. doi: 10.4269/ajtmh.2009.09-0333

Figure 2.

Figure 2

Effects of LAMP reaction volumes on the ability of Anopheles gambiae species diagnosis. One An. gambiae mosquito collected from Iguhu, Kakamega was used as template for all reactions. A, 25 µL final reaction volume; B, 12.5 µL final reaction volume; and C, 8.3 µL final reaction volume. Tube 1: LAMP reaction using An. arabiensis- specific primers; and tube 2: LAMP reaction using An. gambiae -specific primers. A turbid yellow liquid (tube 2) indicates positive amplification, whereas a clear orange-like liquid (tube 1) shows lack of application. No difference in the ability to diagnose An. gambiae species was detected among the LAMP reactions with different volumes. Please note the white precipitate (pyrophosphate ions) generated by the LAMP reaction in the bottom of the tube. This figure appears in color at www.ajtmh.org.