Figure 1.

Induction of IL-10 by supplemented HA hydrogels in vitro. (a) Schematic for the design of a hydrogel capable of delivering a TCR stimulus in association with an HA signal. The “supplemented HA hydrogel” incorporates a crosslinked matrix of thiolated high molecular weight (HMW)-HA, HS, and collagen to which we have added streptavidin and biotinylated anti-CD3 and anti-CD28 antibodies (b-αCD3, b-αCD28) to deliver an activating signal through the TCR. (b) Concentration of IL-10 in supernates taken from T cell cultures 96 hours after either plate-based (left side of graph) or hydrogel-based (right side of graph) activation (cells were cultured on top of the hydrogels for the latter experiments). Supplemented collagen hydrogels that lacked HA were used as a negative control. n = 5 independent experiments.