Figure 3.
Kinetics of release of HA from alginate in vitro. Alginate spheres (2% alginate/10 μL volume/2.2 mm diameter) incorporating 2.5 μg of FITC-labeled 120 kDa HA (closed circles) or FITC-labeled 1.5 × 106 Da HA (open circles) were cultured at 37°C in DPBS Ca/Mg, and the media collected at specific time points for measurement of released HA. Essentially all (98%) of the 120 kDa HA was released over 14 days, whereas less than 5% of the 1.5 × 106 Da HA was released during this time period. Error bars = standard deviations (error bars for the 1.5 × 106 Da group are within the diameter of the open circles). n = 10 spheres for each group.