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Effect of amentoflavone on mRNA expression of proinflammatory genes. (a and b) The mRNA levels of iNOS, COX-2, IL-1β, and TNF-α in RAW264.7 cells pretreated with or without AF (200 μM) for 30 min and then exposed to LPS (1 μg/mL) for 6 h were determined using semiquantitative (a) and real-time (b) PCR. RI: relative intensity. **P < 0.01 compared to the control.
