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. 2013 Jul 21;2013:761506. doi: 10.1155/2013/761506

Figure 3.

Figure 3

Effect of amentoflavone on transcriptional control of inflammatory genes. (a, b, and c) HEK293 or RAW264.7 cells cotransfected with an AP-1-Luc or CREB-Luc plasmid construct (1 μg/mL each) and β-gal (as a transfection control) were treated with amentoflavone (from 0 to 200 μM) in the presence or absence of TNF-α (15 ng/mL), PMA (100 nM), forskolin (2 μM or LPS (1 μg/mL). Luciferase activity was measured using a luminometer. ((d), left panel) Total and phosphorylated levels of the AP-1 family proteins c-Jun and c-Fos in the nuclear fractions of LPS-treated RAW264.7 cells were determined by immunoblotting analyses using specific antibodies. Relative intensity (left panel) was calculated by the DNR Bio-Imaging system (a GelQuant software Ver. 2.7). RI: relative intensity. *P < 0.05 and **P < 0.01 compared to the control.