Table 1.
Primer sequences used in RT-PCR analysis.
| Name | Sequence (5′ to 3′) | |
|---|---|---|
| Real-time PCR | ||
| iNOS | F | GGAGCCTTTAGACCTCAACAGA |
| R | TGAACGAGGAGGGTGGTG | |
| COX-2 | F | CACTACATCCTGACCCACTT |
| R | ATGCTCCTGCTTGAGTATGT | |
| IL-1β | F | GTTGACGGACCCCAAAAAGAT |
| R | CCTCATCCTGGAAGGTCCAC | |
| TNF-α | F | TGCCTATGTCTCAGCCTCTT |
| R | GAGGCCATTTGGGAACTTCT | |
| GAPDH | F | CAATGAATACGGCTACAGCAAC |
| R | AGGGAGATGCTCAGTGTTGG | |
| Semiquantitative PCR | ||
| iNOS | F | CCCTTCCGAAGTTTCTGGCAGCAG |
| R | GGCTGTCAGAGCCTCGTGGCTTTGG | |
| TNF-α | F | TTGACCTCAGCGCTGAGTTG |
| R | CCTGTAGCCCACGTCGTAGC | |
| COX-2 | F | CACTACATCCTGACCCACTT |
| R | ATGCTCCTGCTTGAGTATGT | |
| GAPDH | F | CACTCACGGCAAATTCAACGGCA |
| R | GACTCCACGACATACTCAGCAC |