Figure 5.

APS8 increases the expression of pro-apoptotic proteins and represses the expression of anti-apoptotic proteins. The change in expression of anti- and pro-apoptotic proteins was examined in A549 (A) and SKMES-1 (B) cells after treatment with APS8 (500 nM) or nicotine (1 μM) for 48 h using Human Apoptosis Antibody Array. Fold values relative to untreated cells are given. For presentation proteins are divided into three groups: proteins belonging to the intrinsic pathway, inhibitors of apoptosis (IAPs), and proteins belonging to the extrinsic pathway. Each point is the mean of three independent experiments ± SE. * P < 0.05 compared with control. The insert shows the ratio of main pro-apoptotic and main pro-survival proteins in APS8 and nicotine treated cells.