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. 2013 Jul 22;11(7):2625–2642. doi: 10.3390/md11072625

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) Protein spots marked on the maps were considered differentially expressed as identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results were repeated three times; (B) Western blotting assay to validate the selected proteins identified by 2-DE, including isocitrate dehydrogenase (NAD) subunit alpha (IDH), heat shock protein beta-1 (HspB1), prohibitin, Hsp60, and ubiquinol cytochrome c reductase complex core protein 1 (UQCRC1). T: treatment; C: control, DMSO-treated cells. β-actin was used as the internal control.