Table 3.
Effect of arsenate applied either singly or in combination with phosphate on the oxidative stress elements and anti oxidative enzymes in black gram.
| Control | 100 µM As | 200 µM As | 100 µM As + P | 200 µM As + P | |
|---|---|---|---|---|---|
| Lipid peroxidation (m mol MDA/100 mg fw) |
135.00 ± 13.330 | 139.19 ± 7.870 | 255.60 ± 24.160* | 110.36 ± 2.860 | 122.76 ± 1.430 |
| ELP (%) |
45.15 ± 1.640 | 48.44 ± 0.900 | 54.83 ± 1.860* | 41.75 ± 1.760 | 43.65 ± 0.200 |
| Proline (µM/100 mg fw) |
28.50 ± 6.060 | 34.75 ± 5.630 | 56.00 ± 4.910 | 31.75 ± 7.360 | 35.75 ± 7.940 |
| Catalase (µM H2O2 degraded/mg protein) | 94.31 ± 6.230 | 100.24 ± 2.560 | 71.95 ± 4.650* | 105.22 ± 3.020 | 91.97 ± 2.160 |
| Peroxidase (ΔO.D./mg protein) |
0.02 ± 0.001 | 0.11 ± 0.005* | 0.21 ± 0.019* | 0.04 ± 0.006 | 0.04 ± 0.003 |
| SOD (EU/mg protein) |
1.07 ± 0.257 | 2.07 ± 0.023* | 2.09 ± 0.279* | 1.72 ± 0.103* | 1.95 ± 0.180* |
| APX (nM ascorbate oxidized min−1 mg−1 protein). |
3.60 ± 0.011 | 4.10 ± 0.006* | 4.30 ± 0. 011* | 3.90 ± 0. 017* | 4.00 ± 0.017* |
P = 40 ppm K2HPO4; values are means of three replicates ± SE *Data significant at P < 0.05. Multiple comparisons versus control group (Holm Sidak method) overall significance level = 0.05.