Table 1.

Primers used in this study (5′ → 3′).

Species	Forward primer	Reverse primer	Reference
Common for FLA	CGCGGTAATTCCAGCTCCAATAGC	CAGGTTAAGGTCTCGTTCGTTAAC	Tsvetkova et al., 2004 [7]
Acanthamoeba spp.	GGCCCAGATCGTTTACCGTG	TCTCACAAGCTGCTAGGGAGTCA	Schroeder et al., 2001 [22]
N. fowleri	CAAACACCGTTATGACAGGG	CTGGTTTCCCTCACCTTACG	Schild et al., 2007 [23]
B. mandrillaris	CGCATGTATGAAGAAGACCA	TTACCTATATAATTGTCGATACCA	Booton et al., 2003 [24]
Sappinia	TCT GGT CGC AAG GCT GAA AC	GCA CCA CCA CCC TTG AAA TC	Qvarnstrom et al., 2009 [10]

FLA: Free-living amoebae.