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. 2013 Jul 22;2013:408573. doi: 10.1155/2013/408573

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Copyright © 2013 Erika Vyskočilová et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Age-related changes in the hepatic CBR1 and GSTA protein expression normalized to the amount of β-actin (a). Interindividual differences in protein expression of GST and CBR1 in rat liver (b). Cytosolic fractions (4 μg of protein/lane) of 6-week-old (J1–J6) and 21-month-old (S1–S6) rats were subjected to SDS-PAGE with subsequent immunoblotting. Blots were then reacted with rabbit polyclonal anti-CBR1 antibody or goat polyclonal anti-GSTA antibody. Protein bands were marked by corresponding secondary antibodies using a chemiluminescent detection with an alkaline phosphatase substrate. Protein expression was normalized to the amount of the loading control β-actin using mouse monoclonal anti-beta-actin antibody. GSTA = alpha-glutathione S-transferase; CBR1 = carbonyl reductase 1.