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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Gastroenterology. 2013 Jan 18;144(5):1107–1115.e3. doi: 10.1053/j.gastro.2013.01.022

Figure 2.

Figure 2

Knockdown of gpc1 in zebrafish leads to developmental biliary defects. (A, Ai) Right lateral views of 5 dpf zebrafish larvae after ingestion of PED-6, showing a more intense and larger gallbladder in (A) control than in (Ai) the gpc1 morphant. (B) Quantification of PED-6 uptake in control and gpc1 morphant larvae, showing a highly significant (P < .0001, χ2) decrease in gallbladder intensity in the gpc1 morphants. (C and D) Confocal projections of whole-mount cytokeratin immunostaining of livers from 5 dpf (C) control and (D) gpc1 morphant, showing decreased number and complexity of the ducts in panel D. (E and F) Confocal projections of gallbladders stained for cytokeratin and counterstained with 4’,6-diamidino-2-phenylindole (DAPI), with cells noted by white dots. Note that there are more cells in panel E.