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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Cell Rep. 2013 Jul 3;4(1):66–75. doi: 10.1016/j.celrep.2013.06.012

Figure 5. Endogenous FGF14 regulates vesicular recycling and short-term synaptic plasticity.

Figure 5

A. Confocal images from cultured cerebellar neurons expressing GFP (left), scrambled control shRNA (middle), or FGF14 shRNA (right) that were loaded with FM4-64 by a 90 sec depolarization using 90 mM KCl. Scale bar, 5 µm. B. The distribution of FM4-64 puncta per synapse within a 45×45 µm2 region of interest (ROI) in neurons transfected with control GFP (black, n=81), scrambled control shRNA (gray, n=49), or FGF14 shRNA (blue, n=87). The data for each group were fit to a Gaussian distribution. C. Average puncta per 45×45 µm2 ROI for neurons over-expressing control GFP, scrambled control shRNA, or FGF14 shRNA. D. Representative EPSCs evoked by a paired-pulse protocol (indicated in inset; 80 ms interstimulus interval) when the presynaptic granule cells expressed GFP control (black), scrambled control shRNA (gray), or FGF14 shRNA (blue). E. Averaged paired-pulse ratio (amplitude of the second EPSC divided by the amplitude of the first EPSC). **p<0.01 versus Control.