Table 1.
Summary electrophysiology data for CaV2.1 and CaV2.2 channel activation and inactivation in HEK293 cells
| V1/2 (mV) | K | n | ||
|---|---|---|---|---|
| Activation | ||||
| CaV2.1 | Control | −11.5 ± 0.9 | 5.9 ± 0.2 | 31 |
| FGF14WT | −12.5 ± 0.5 | 5.4 ± 0.2 | 44 | |
| CaV2.2 | Control | −4.8 ± 0.8 | 5.6 ± 0.2 | 24 |
| FGF14WT | −5.2 ± 0.8 | 5.5 ± 0.2 | 25 | |
| Inactivation | ||||
| CaV2.1 | Control | −19.3 ± 0.8 | 17.5 ± 1.4 | 23 |
| FGF14WT | −18.2 ± 2.3 | 15.1 ± 0.6 | 20 | |
| CaV2.2 | Control | −18.4 ± 1.5 | 14.8 ± 1.3 | 15 |
| FGF14WT | −20.0 ±1.5 | 14.1 ± 0.6 | 14 |
Activation kinetics were obtained from fits with a Boltzmann equation of the form: G=Gmax/[1 + exp(V − V1/2)/k], where Gmax is the extrapolated maximum Ca2+ conductance, V is the test voltage, V1/2 is the half-activation voltage, and k is the slope factor. Steady-state inactivation were obtained from fits with a Boltzmann relationship, I/Imax = (1 + exp((V − V1/2)/k))−1.