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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Mol Microbiol. 2008 Aug 27;70(2):379–395. doi: 10.1111/j.1365-2958.2008.06417.x

Figure 6. Crg2 functions upstream of Gpa1 in cAMP signaling regulation.

Figure 6

A) Mating assays for the following strains were cocultured on MS medium for 7 days in the dark at 25°C and photographed. H99 × KN99a, α pka1 (JHK7) × apka1 (JKH74), α gpa1 (YSB83) × KN99a, α pde1 (JKH63) × KN99a, α PGPD1-CRG2 (CDX154) × KN99a, α PGPD1-CRG2 pka1 (CDX186) × aPGPD1-CRG2 pka1 (CDX187), α PGPD1-CRG2 gpa1 (CDX188) × KN99a, α PGPD1-CRG2 pde1 (CDX189) × KN99a. B) capsule production was visualized by India ink staining after growth on DME medium for 3 days. The following strains were used in this assay; H99, α gpa1 (YSB83), α pka1 (JHK7), α pde1 (JKH63), α PGPD1-CRG2 (CDX154), α PGPD1-CRG2 gpa1 (CDX188), α PGPD1-CRG2 pka1 (CDX186), α PGPD1-CRG2 pde1 (CDX189). C) Melanin production was assayed on Niger seed media. The same set of strains as in B) were used for melanin assays. Plates were incubated at 37°C. D) Melanin production was assayed on Niger seed media containing 1% glucose and without (upper) or with (lower) addition of 1 mM cAMP. H99, crg2 (CDX50), gpa1 (YSB83), PGPD1-CRG2 (CDX154),PGPD1-GPA1Q284L(CDX156-2), crg1 crg2 (CDX115), and crg2 crg3 (CDX193) strains were used in this assay. Plates were incubated at 30°C. Melanin levels produced by the strains tested in panels C and D were photographed after incubation for 3 days.