Figure 1.

LCI quantifies HSF1 hPSC colony mass. (A) Simplified schematic of the live cell interferometer (LCI) used to obtain dynamic mass distributions of living cells. LCI uses a standard light microscope with a Michelson interferometer between the microscope objective and the temperature and CO₂-controlled observation chamber. The interferometer compares lights that pass through cells to light that passes undisturbed through a reference chamber. (B) LCI directly measures the phase shift of light due to its interaction with the material inside a cell. This is converted to cell dry mass using the measured relationship between light phase shift and material density, yielding the mass distribution within each individual colony. Scale bar in panel B is 20 μm.