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. 2013 Aug 6;105(3):593–601. doi: 10.1016/j.bpj.2013.06.041

Figure 3.

Figure 3

Average mass accumulation rate for untreated and retinoic acid (RA)-exposed hPSC colonies. (A) Growth rate versus initial mass for untreated and RA-exposed hPSC colonies shows an exponential growth rate for mass accumulation across five orders of magnitude in mass. RA-treated colonies show similar mass accumulation rates to untreated colonies. Error bars show the uncertainty in measured growth rate calculated as the SD of the slope of the biomass versus time measurements. Error bars appear uneven due to the logarithmic scale. (B) Histogram of untreated control colony growth rate measurements (normalized by initial mass to provide specific growth rate) with the log-normal curve (red) showing the estimated distribution of specific growth rates that was used to compute doubling time. (C) Doubling time from cell counting is unable to distinguish cell proliferation rates between control and RA-exposed hPSCs during 4–6 days of growth. Doubling time for cell mass shows a decrease of ∼15% with RA treatment (based on measurements of 658 control, 227 RA day-4, and 62 RA day-6 colonies). ctrl = control; RA4 = 4 days of RA treatment, RA6 = 6 days of RA treatment. Error bars show ± SE ∗p < 0.05, ∗∗p < 0.001.